Product overview
The SLiM1000 scanning light field microscope is a new generation of live-cell 3D imaging tools with high speed, high resolution, and comprehensive observation from macroscopic to microscopic.
Three-dimensional imaging of zebrafish embryonic development |
Observation of three-dimensional neural response of Drosophila larvae |
Diffraction limit resolution product features
- 90Hz body imaging
- Four-channel imaging
- Adaptive aberration correction improves the signal-to-noise ratio across the board
- Large NA objectives support oblique illumination to reduce phototoxicity and improve signal-to-noise ratio
| Sxian’weijLim 1000 Imaging microscopy systems | Laser scanning confocal microscopy systems | Transfer to confocal imaging microscopy systems | |
| Technical specifications | Configure the objective lens:60X/1.42NA,0.15WD,OIL(UPLXAPO60XO) | Configure the objective lens: 60X/1.42NA,0.15WD,OIL(UPLXAPO60XO) | Configure the objective lens:CFI Apochromat TIRF 60XC/NA 1.499 Oil |
| Adaptive optics | Supports correction of optical aberrations that are spatially inconsistent with the presence of samples and systems | Not supported | Not supported |
| Landscape resolution（500nmwavelength） | Under the adaptive correction technology, it can approach the diffraction limit resolution, about 250 nm | Without adaptive correction techniques, transparent samples can achieve diffraction-limited resolution 140nm | Without adaptive correction techniques, transparent samples can achieve diffraction-limited resolution 120nm |
| Axial resolution(500nmwavelength) | Under the adaptive correction technology, it can approach the diffraction limit resolution, about 320 nm | Without adaptive correction techniques, transparent samples can achieve diffraction-limited resolution 520nm | Without adaptive correction techniques, transparent samples can achieve diffraction-limited resolution 472 nm |
| Field of view | Single imaging range, the maximum can reach 220μmx220μmx15μm | Single imaging range, the maximum can reach 220μmx220μmx0.52μm | Single imaging range, the maximum can reach 220μmx220μmx0.47μm |
| Acquisition speed | Adaptive imaging up to 90Hz (limited by camera frame rate) | Fastest imaging up to 2Hz (2048×2048 pixels) | Fastest imaging up to 30Hz (1068×1068 pixels) |
| Data throughput | Over 5 billion voxels per second, supporting rapid collection of more than 5,000 individuals | Close to 8.4 million voxels per second | Close to 78 million voxels per second |
| Color channels | Lasers of different wavelengths (405nm, 488nm, 561nm, 640nm, etc.) can be selected to excite and acquire at the same time. | Lasers of different wavelengths (405nm, 488nm, 561nm, 640nm, etc.) can be selected to excite and acquire at the same time. | Lasers of different wavelengths (405nm, 488nm, 561nm, 640nm, etc.) can be selected to excite and acquire at the same time. |
| Oblique excitation | For objectives above 1.0NA, the illumination module supporting oblique illumination is expanded to reduce background fluorescent phototoxicity. | No … | No … |
| cell culture | Supports live-cell workstation operation, providing conditions for live-cell imaging. | Supports live-cell workstation operation, providing conditions for live-cell imaging. | Supports live-cell workstation operation, providing conditions for live-cell imaging. |
